Inverter

Part:BBa_K2055015:Design

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 258
    Illegal XhoI site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 179
    Illegal BsaI.rc site found at 395


Design Notes

LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.


Source

Composite part

References